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KMID : 0360319920240040504
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Journal of Korean Cancer Research Association
1992 Volume.24 No. 4 p.504 ~ p.515
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Effect of Retinoic Acid, B-Carotene, and Ascorbic Acid on the Mutagenicity of Some Anticancer Antibiotics
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Jeong Hye-Won
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Abstract
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Some anticancer antibiotics such as daunomycin, mitomycin C, and bleomycin are known to exert their anticancer effect by damaging host DNA through the generation of free radicals. In this study, effects of retinoic acid, β-carotene, ascorbic
acid
on
the mutagenicity of the anticancer antibiotics were investigated by using preincubation Salmonella/microsome assay with Salmonella typhimurium TA102 strain, which is particularly useful for the detection of oxidative mutagens. Appropriate
concentrations
of the antibiotics being incorporated into the preincubation mixture and minimal glucose agar were determined by checking MICs using Microbiology System 2(MS-2, Abbott Co., U. S.A.). Alteration in the mutagenicity of the anticancer antibiotics
was
observed when equivolumes of 1mM, 0.1mM, and 0.01mM of retinoic acid and β-carotene, and 10mM, 1mM, and 0.1mM of ascorbic acid was added to the preincubation mixture. And bleomycin in order.
Mutagenicity of daunomycin was significantly suppressed by retinoic acid (1mM, 0.1mM, and 0.01mM), β-carotene(1mM and 0.1mM), and ascorbic acid(10mM and 1mM). Mutagenicity of mitomycin C was suppressed only by 10mM of ascorbic acid. Mutagenicity
of
bleomycin was suppressed only by 1mM of retinoic acid. On the contrary, 0.1mM and 0.01mM of both reitnoic acid and β-carotene, and 0.1mM of ascorbic acid potentiated the mutagenicity of bleomycin significantly.
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KEYWORD
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